Sure. If you want to reuse code, you simply give it a name, and use that name twice.

sub handle_my_request { 
   ...
}
for my $route ('/myaction1/:param1', '/myaction2/:param2') {
    get $route, \&handle_my_request;
}
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Most frameworks also allow you to write the code as methods in a controller class (see for example Mojolicious::Guides::Growing).