I first thought that this question would target people who specifically know how Glade works when it generates Perl code, because I suppose this is a bug with Glade. Here goes the entire code for app.pl:

use Wx 0.15 qw[:allclasses];
use strict;
1;
package main;
use MyFrame8;
unless(caller){
    local *Wx::App::OnInit = sub{1};
    my $app = Wx::App->new();
    Wx::InitAllImageHandlers();
    my $frame_9 = MyFrame8->new();
    $app->SetTopWindow($frame_9);
    $frame_9->Show(1);
    $app->MainLoop();
}
[download]

MyFrame8.pm is also generated by Glade and it has a bit more code that makes the frame. Still, there's no OnInit in there. The only OnInit is the one above, also generated by Glade. Here's MyFrame8.pm then:

use Wx 0.15 qw[:allclasses];
use strict;
package MyFrame8;

use Wx qw[:everything];
use base qw(Wx::Frame);
use strict;

# begin wxGlade: ::dependencies
# end wxGlade

sub new {
    my( $self, $parent, $id, $title, $pos, $size, $style, $name ) = @_
+;

    $parent = undef              unless defined $parent;
    $id     = -1                 unless defined $id;
    $title  = ""                 unless defined $title;
    $pos    = wxDefaultPosition  unless defined $pos;
    $size   = wxDefaultSize      unless defined $size;
    $name   = ""                 unless defined $name;

# begin wxGlade: MyFrame8::new

    $style = wxDEFAULT_FRAME_STYLE 
        unless defined $style;

    $self = $self->SUPER::new( $parent, $id, $title, $pos, $size, $sty
+le, $name );
    $self->{panel_1} = Wx::Panel->new($self, -1, wxDefaultPosition, wx
+DefaultSize, );
    $self->{label_1} = Wx::StaticText->new($self->{panel_1}, -1, "labe
+l_1", wxDefaultPosition, wxDefaultSize, wxALIGN_CENTRE);
    $self->{Test} = Wx::Button->new($self->{panel_1}, -1, "Test");

    $self->__set_properties();
    $self->__do_layout();

# end wxGlade

    return $self;
}


sub __set_properties {
    my $self = shift;

# begin wxGlade: MyFrame8::__set_properties

    $self->SetTitle("frame_9");
    $self->SetSize(Wx::Size->new(400, 300));

# end wxGlade
}

sub __do_layout {
    my $self = shift;

# begin wxGlade: MyFrame8::__do_layout

    $self->{sizer_9} = Wx::BoxSizer->new(wxVERTICAL);
    $self->{sizer_10} = Wx::BoxSizer->new(wxVERTICAL);
    $self->{sizer_11} = Wx::BoxSizer->new(wxVERTICAL);
    $self->{sizer_10}->Add($self->{label_1}, 1, wxALIGN_CENTER_HORIZON
+TAL, 0);
    $self->{sizer_11}->Add(0, 78, 0, wxADJUST_MINSIZE, 0);
    $self->{sizer_11}->Add($self->{Test}, 0, wxALIGN_CENTER_HORIZONTAL
+, 0);
    $self->{sizer_10}->Add($self->{sizer_11}, 1, wxALL|wxEXPAND, 0);
    $self->{panel_1}->SetAutoLayout(1);
    $self->{panel_1}->SetSizer($self->{sizer_10});
    $self->{sizer_10}->Fit($self->{panel_1});
    $self->{sizer_10}->SetSizeHints($self->{panel_1});
    $self->{sizer_9}->Add($self->{panel_1}, 1, wxEXPAND, 0);
    $self->SetAutoLayout(1);
    $self->SetSizer($self->{sizer_9});
    $self->Layout();

# end wxGlade
}

# end of class MyFrame8

1;
[download]