open my $fh_in , '<', 'test1.txt' or die "Can not open file test1.txt:
+ $!";
open my $fh_out, '>', 'test2.txt' or die "Can not open file test2.txt:
+ $!";
while (<$fh_in>) {
    s/\s+/\t/g;    
    print $fh_out "$_\n";
}
close $fh_out;
close $fh_in;

##</code><code>##

MGI:1918918                    381629                         0610007C21Rik             5            syntenic            51374                          C2orf28                   2p23.3                    B
MGI:1918917                    71667                          0610007L01Rik             5            syntenic F          55069                          C7orf42                   7q11.21                   C
MGI:1923501                    76251                          0610007P08Rik             13           syntenic B3         375748                         C9orf102                  9q22.32                   M
MGI:1915571                    58520                          0610007P14Rik             12           syntenic            11161                          C14orf1                   14q24.3                   B