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Hi I have a fasta file containing a number of sequences. I would like to extract only the sequences that are of a specific length (lets's say >10 nt). The sequences are wrapped with new lines, so when trying to read into an array, each line becomes an element. How can I removed these? I have tried join, but it also removes the newline between sequences and puts all of it into single string. How do I separate each sequence into an element or string?

The file looks something like this:

>NM_001 Homo sapiens ADA2 (CECR1) GATCCAA >NM_002 Homo sapiens IKBKG GGAGGTCTTTAGCTTTAGGGAAACCC
Output should be only the second sequence in this case.
#!/usr/bin/perl -w #open the fastfile Genes.fasta open (GENES, "Genes.fasta") or die "Could not open file"; chomp (@seq = <GENES>); $seq = join ("\n", @seq); $lengthseq = length $seq; #min length of the seq $minlength = 10; #if length is over a certain size, print if ($lengthseq > $minlength){ print $seq; } else {print "No sequence is over $minlength;" }

In reply to Size of sequences in fastafile by Sofie

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