#!/usr/bin/perl
use strict;
use warnings;
use Bio::Tools::pICalculator;
use Bio::SeqIO;

@ARGV == 1 or die "Usage: perl $0 fasta_file_name\n";

my $in = Bio::SeqIO->new( -file => $ARGV[0] ,-format => 'Fasta' );

my $calc = Bio::Tools::pICalculator->new(-places => 6);

while ( my $seq = $in->next_seq ) {
	 $calc->seq($seq);
	 my $iep = $calc->iep;
	 print join("\t", $seq->id, $iep), "\n";
}