Processing data... Original.txt Writing output...Use of uninitialized value $metabolite[44] in string at G:\Metabolomics\Programming\PERL\Ratio Test\ratio test (updated 12 feb 1 4).pl line 101, line 1 (#1) (W uninitialized) An undefined value was used as if it were already defined. It was interpreted as a "" or a 0, but maybe it was a mistake. To suppress this warning assign a defined value to your variables. To help you figure out what was undefined, perl will try to tell you the name of the variable (if any) that was undefined. In some cases it cannot do this, so it also tells you what operation you used the undefined value in. Note, however, that perl optimizes your program and the operation displayed in the warning may not necessarily appear literally in your program. For example, "that $foo" is usually optimized into "that " . $foo, and the warning will refer to the concatenation (.) operator, even though there is no . in your program. Writing output...Uncaught exception from user code: Error couldn't create new Directory at G:\Metabolomics\Programming\PERL\ Ratio Test\ratio test (updated 12 feb 14).pl line 98, line 2. at G:\Metabolomics\Programming\PERL\Ratio Test\ratio test (updated 12 feb 14).p l line 98 Press any key to continue . . . #### push(@data1, $columns[1]); push(@data2, $columns[2]); push(@data3, $columns[3]); #### push (@data[j], $columns[j]);