#!/usr/local/bin/perl open (FASTAFILE, 'human_fold_25.txt'); $sequence = ' '; $Ccount = 0; $Gcount = 0; $identifier = ' '; while ($line = ) { chomp $line; if ( $line =~ /^>/ ) { $identifier = $line; } elsif ( $line =~ /^\./ ) { next; } elsif ( $line =~ /^\(/ ) { }else { $sequence = $line; } } $sequencelength = length ($sequence); @nucleotides = split ( '' , $sequence ); foreach $nuc (@nucleotides) { if ( $nuc eq 'G') { $Gcount = $Gcount + 1; } elsif ( $nuc eq 'C') { $Ccount = $Ccount + 1; } } $GCcontent = ((( $Gcount + $Ccount )/ $sequencelength ) * 100); close (FASTAFILE); print "$identifier", ", ", "$GCcontent\n"