APOL4 protein_coding CYP2C8 protein_coding NAALADL2 protein_coding NANOS3 protein_coding C20orf204 protein_coding MIR429 miRNA MIR200A miRNA MIR200B miRNA I have a file named "gene.txt", which looks like this GeneName GeneType APOL4 protein_coding CYP2C8 protein_coding NAALADL2 protein_coding NANOS3 protein_coding C20orf204 protein_coding MIR429 miRNA MIR200A miRNA MIR200B miRNA #### #!/usr/bin/perl -w use strict; open FILE1, "/Desktop/gene.txt" or die; my %hash; while (my $line=) { chomp $line; # print $line,"\n"; (my $word1,my $word2) = split /:/, $line; $hash{$word1} = $word2; } # Print hash for testinf purposes while ( my ($k,$v) = each %hash ) { # print "Key $k $v\n"; @keys = keys(%hash); print @keys; }