my $url = 'http://www.csbio.sjtu.edu.cn/cgi-bin/MEMBRAIN.cgi'; my $mech = WWW::Mechanize->new (timeout=>1000); $mech->get($url); $mech->submit_form ( form_number => 1, fields => { #textarea name | value S1 => ">$id\n$seq", #Radio button name | prediction type R4 => TOPpre, #Radio button SIGNAL | I do not know if I have a signal peptide R2 => SignalYES, #Radio button ORGANISM | Human, Plant, Animal, OEuk, Gpos, Gneg, R3 => Human, #Fill in email email => 'testmail@gmail.com' } );