use WWW::Mechanize(); while(<>) { if($_=~/^>(.*)/) { my $id=$1; my $seq=<>; chomp $seq; print "$id\n"; my $url = 'http://www.csbio.sjtu.edu.cn/bioinf/MemBrain/'; #what I see in the browser, as you said my $mech = WWW::Mechanize->new(); $mech->get($url); $mech->submit_form ( form_name => 'myform', fields => { #textarea name | value S1 => ">$id\n$seq", #Radio button one with value (parameter 1) R4 => 'TOPpre', #Radio button one with value (parameter 2) R2 => 'SignalYES', #Radio button one with value (parameter 3) R3 => 'Human', #Fill in email (parameter 4) email => 'myemail@gmail.com' } ); } }