$ perl -Mstrict -Mwarnings -E '
my @seqs  = qw{ ATGC TGCT GCTA CTAA TAAC };
my $match =  q{GTCA};

foreach my $seq ( @seqs )
{
    my @posns;
    my $diff = $seq ^ $match;
    my $nMismatches = () = $diff =~ m{([^\0])(?{ push @posns, pos $diff })}g;
    say qq{$seq : $match -> $nMismatches at @posns};
}'
ATGC : GTCA -> 3 at 1 3 4
TGCT : GTCA -> 3 at 1 2 4
GCTA : GTCA -> 2 at 2 3
CTAA : GTCA -> 2 at 1 3
TAAC : GTCA -> 4 at 1 2 3 4
$