use warnings; use strict; my $maf = "input.maf"; open (IN1, "input.maf") or die; open (OUT1, ">m2.maf") or die; open (OUT2, ">m2_most-cons.gff") or die; while (my $line1 = ) { chomp $line1; if ($line1 =~ /^s\sficAlb2/) { my @columns = split(/\t/, $line1); my $start = $columns[2] + 1; my $end = $columns[2] + $columns[3]; my $chrom = $columns[1]; my $mafBlock = qx(maf_parse --start $start --end $end $maf); print OUT1 "$mafBlock1\n"; my $phastCons = qx(phastCons --target-coverage 0.3 --expected-length 45 --rho 0.31 --most-conserved m2.gff --seqname $chrom --msa-format MAF m2.maf nonconserved-all-4d.mod); print "$phastCons\n" open (IN2, "m2.gff") or die; while (my $line2 = ) { if ($line2 =~ /^fic/) { print OUT2 "$line2\n"; } } } open (OUT1, ">m2.maf") or die; close IN2; } close IN1; close OUT1; close OUT2; exit;