500 (Internal Server Error) Not a SCALAR reference

Client-Date: Mon, 04 Mar 2002 20:42:13 GMT

##</code><code>##

#!/usr/bin/perl -w
use strict;
use Getopt::Std;
use LWP::UserAgent;
use HTTP::Request::Common qw(POST);
use vars qw($ua);

my %opts;

getopts('hp:d:i:o:e:v:b:F:', \%opts);

my $PROGRAM = $opts{'p'};
my $DB      = $opts{'d'};
my $INFILE  = $opts{'i'};
my $OUTFILE = $opts{'o'};

my $ESCORE;
my $V;
my $B;
my $F;

if (defined $opts{'e'}) {
    $ESCORE=$opts{'e'};
} else {
    $ESCORE=0.01;
}
if (defined $opts{'v'}) {
    $V=$opts{'v'};
} else {
    $V=10;
}
if (defined $opts{'b'}) {
    $B=$opts{'b'};
} else {
    $B=10;
}
if (defined $opts{'F'}) {
    $F=$opts{'F'};
} else {
    $F='T';
}

#--------------------------------------------------------------
my $seq="GCAGCAGGTCGGTAGGCGGGAAATGGCGACTGGCTGAAGGAGCTGGTTCTGTTGCTGCTGCGGGGTAAGCGGGAAAGACACCACACA";

$DB = 'baygenomicsdb';
$ua = LWP::UserAgent->new;

my $req = HTTP::Request->new(POST => 'http://baygenomics.ucsf.edu/db/cgi-bin/blast/blast.cgi');
$req->content_type('form-data'); #also tried 'application/x-www-form-urlencoded'

$req->content( [ PROGRAM => $PROGRAM, DATALIB => $DB,
                 SEQUENCE => $seq,
                 EXPECT  => $ESCORE,
                 DESCRIPTIONS => $V, ALIGNMENTS => $B ]);

print $ua->request($req)->as_string;


##</code><code>##

$ ./test-post.pl -p blastn -d hs_all.fa -i test.in -o test.out