#!/usr/bin/perl -w
use strict;
use Getopt::Std;
use LWP::UserAgent;
use HTTP::Request::Common qw(POST);
use HTML::TreeBuilder;
use HTML::FormatText;
use vars qw($ua);

my %opts;

getopts('hp:d:i:o:e:v:b:F:', \%opts);

if (defined $opts{'h'}) {
print<<"HERE";
Usage:  Very much like commandline blast:
        -p  program
        -d  database (though it has to be the name the the webserver knows
                      ie, NOT /data/db/ncbi/something.)
        -i  input file (must be fasta with empty line between seqs)
        -o  output file
        -e  escore cutoff (default 0.01)
        -v and -b alignments and oneline descriptions (default 10)
        -h  this message
Those are currently the only arguments it knows.
Note that you must specify all of -p, -d, -i, and -o
And I am not going to do any sanity checking--arguments better
be spelled right

HERE
die;
}
if (! (defined $opts{'p'} and defined $opts{'d'} and
       defined $opts{'i'} and defined $opts{'o'}) ){
    die "you must specify all of -p, -d, -i, and -o\n";
}

my $PROGRAM = $opts{'p'};
my $DB      = $opts{'d'};
my $INFILE  = $opts{'i'};
my $OUTFILE = $opts{'o'};

my $ESCORE;
my $V;
my $B;

if (defined $opts{'e'}) {
    $ESCORE=$opts{'e'};
} else {
    $ESCORE=0.01;
}
if (defined $opts{'v'}) {
    $V=$opts{'v'};
} else {
    $V=10;
}
if (defined $opts{'b'}) {
    $B=$opts{'b'};
} else {
    $B=10;
}

#--------------------------------------------------------------
my $seq;
my $dotheblast =1;
open OUT, ">$OUTFILE" or die "couldn't open $OUTFILE: $!\n";
open IN,    $INFILE   or die "couldn't open $INFILE: $!\n";
while (<IN>) {
    if (/^>/) {
        $seq = $_;
        $dotheblast=1;
    } elsif (/^[ATGCNX]+$/i) {
        $seq .= $_;
    } elsif ($dotheblast == 1 and $seq ne "") {

        $ua = LWP::UserAgent->new;

        my $data = [ PROGRAM => $PROGRAM,
                     DATALIB => $DB,
                     SEQUENCE => $seq,
                     EXPECT  => $ESCORE,
                     DESCRIPTIONS => $V,
                     ALIGNMENTS => $B,
                   ];

        my $action = 'http://scain/blast/blast.cgi';
        my $req = $ua->request(POST($action, $data));

        while (!$req->is_success) { # if it isn't available now, wait and try again.
            print "sleeping...\n";
            sleep 600;
            $req = $ua->request(POST($action, $data));
        }

        if ($req->is_success) {
            my $result = $req->content;
            my $tree = HTML::TreeBuilder->new_from_content($result);
            my $formatter = HTML::FormatText->new(leftmargin => 0, rightmargin => 80);
            print OUT $formatter->format($tree);
        }

        $seq="";
        $dotheblast=0;
    }
}
close IN;
close OUT;