open( FASTAFILE, $ARGV[0] ); while () { if ( /^>/ && $seqflag == 1 ) { push ( @sequences, $fasta ); $fasta = ""; $fasta = $_; } elsif (/^>/) { $fasta = $_; $seqflag = 1; } else { $fasta .= $_; } } push ( @sequences, $fasta ); #then iterate @sequences to run over BLAST #### # script 1: create the index use Bio::Index::Fasta; # using fasta file format $Index_File_Name = shift; $inx = Bio::Index::Fasta->new( -filename => $Index_File_Name, -write_flag => 1); $inx->make_index(@ARGV); # script 2: retrieve some files use Bio::Index::Fasta; $Index_File_Name = shift; $inx = Bio::Index::Fasta->new($Index_File_Name); foreach $id (@ARGV) { $seq = $inx->fetch($id); # Returns Bio::Seq object # do something with the sequence }