use strict;
my $filename = "File_Name_One";
my $letter = "A";

my $dbh = DBI->connect("DBI:ODBC:databasename", "",""))

my $firstvar = $dbh->quote($filename);
my $secondvar = $dbh->quote($letter);

my $statement = "Insert into tableOne (filename, letter) 
values ($firstname, $letter)";