my $header_line = <DATA>;

my %count;

my @chars = ( qw/F G S/ );

while (my $line = <DATA> ) {
    eval "\$count{$_} += \$line =~ tr/$_/$_/,1" 
    or die $@ foreach @chars;
}

print "There are $count{$_} occurrences of $_\n"
foreach sort keys %count;

__DATA__
Sample header hine
FDIELSIGCOXLSAGICK\n
FDIELSIGCOXLSAGICK\n