use strict; use warnings; use Data::Dumper; my %seq_count; local $/ = '>gb|'; while (my $seq = ) { $seq =~ s/>gb\|//; next if ! length($seq); # get sequence id my ($seq_id) = $seq =~ /\|:(.*)/; # remove anything other than sequence characters $seq =~ s/^.*\n|\n//g; # build hash of sequence count $seq_count{$seq_id} = length($seq); } print Dumper(\%seq_count); __DATA__ >gb|AE008689|:2073051-2073458, Atu4895 TTGGTCACATATTTTCGCTATTCGAAACCCAAATATCCCTGCGGAACACGTTTTATTGGAGCCGCCGTTT TGCAGTTGCTCGGTGCTGGATTCTTGGCGTTCGTCTTTTGTCTGCTGGATGGGCTCACTGCAAAACCAAC GATAATTCTCGGTCAGTTTGTATCATGCCTAGTGGGGAGCGTCGCCGGCTTTCATTTCGTGGCTTTTCGT CGCCCAGGCACGGACGGCCAACTTTACCTTATCGCGACGTCGCTCTTGGCATTTGGGACCCATTATTGGC TGGTGTCATATTCATTACCTGACCTTTTGCTAGCAAGATTGATTTCAGGATTTGGATCGGGTGTGGTTGT TGCAGGAACTTTCCGACGTCGCTTTCTGGAAAATCCGGTAATTCCCTGCGTTCGATAG >gb|AE008689|:c2074151-2073549, Atu4896 GTGTTTGGACCATATTTTCTGTGCAAACTAAACGATGACATAGGGCGATTTTTAGTGGCGGACAAATACA GACTTCCCGAAGAGTTTTTTACCACTCGGTTTCTCGTTAGACGCATCGTACCCACAGACGCTGAAGCTAT TTTCGAAGGGTGGAACACCGATCCCGAGGTGACGAAGTACCTGACGTGGAAACCCCACTCCGAGCTTGGC CAGACACAGCGGGCGATTGAAGAAAATTATAGTGCGTGGAATGCAGGTACATCGTTTCCAGCTGTCATCT GCCATCGCGAACGGCCACATGAACTAATCGGCCGTATTGATGCACGTCCGATGGGCCACAAGGTCTCTTA CGGGTGGCTTGTCCGAAGAACCTGGTGGGGCCGGGGTGTTGCAAGCGAGGTCGTTCAACTCGCTGTAGAA CACGCGTTATCGCATCCGCGCATCTTTCGCACCGAAGCATCCTGCGACGTTCTGAACACGGCGTCAGCAA GAGTGATGGAAAAAGTAGGGATGACAAAGGAGGCCGTGCTTCGACGGTACCTTTTTCACCCCAATTTTTC GAATATGCCGCGAGACGCCTTCCTGTATTCCAAGGTACGTTAA >gb|AE008689|:c2074749-2074345, Atu4897 ATGAAACATACCATCGCAGTTCTCGGCCTGATCACCTTCTCCAGCCCGGCCTTCGCAGCATCGTGCGAGA AAAACTTCACCGTCTCAGGCGTACCGATGGTCACGGCTGTCTCTTACAAATCCTTTCAGGAACTGCCGAA AGCCAAAGCACCAGCTGTCCTTCAAAAGCTCGCCCAGGCCGTCGCGGCAGAAGGTTTTTCAGGTATCCAG ATCAACAAGGCACTGTCGTCAATCGATGCCCATCAGGAAACCAGCGGAAGTGGCAGGATTCAGACGCTGC GGGTTGTCGCCCGCCAGAAAGGCGCCGCTGTCCGGATCGATGCTGTCTTCAATATTCAGGCAGGACAGAT CGCCGACAAAGACGTCATCCGCAAGGGCATCTGCGACATCATAAAAGGCGCGTAA #### $VAR1 = { 'c2074151-2073549, Atu4896' => 603, '2073051-2073458, Atu4895' => 408, 'c2074749-2074345, Atu4897' => 405 };