open(INPUTFILE, "$database") or die "cannot open file: $!"; 
		    
while (<INPUTFILE> ) {
 
 my $path_check=$_; #It should have all the entry from the db text file
 chomp($path_check);
 
# $input{'nfirst'}) is one of the parameters from the form so it  the value of $input{'slast'}

  if( $path_check eq $input{'nfirst'}) || ($path_check eq $input{'slast'}) ){
				      
    print "<b>Dont Print entry to db text file and exit the loop here</b>"; 
		                
}else{
	print "Print New Value(s) to db text file here and exit the loop</b><br>";}   
		      
		      }
close(INPUTFILE);

_db sample

D:/myfiles/Test/
E:/pics/cgi-bin/new/
C:/temp/
D:/new/files/
F:/new/more/
C:/test/cgi-bin/ac/^ok
C:/new/files/   # This line shouldn't be allowed here, it is duplicated.