use strict; use warnings; use Data::Dumper; { local $/; $_ = ; } my %sequences = map { $_->[0], $_->[1] } sort { length $a->[1] <=> length $b->[1] } map { [split m{\n}] } m { > ( [^\n]+ \n [^\n]+ ) }gx; print Dumper(\%sequences); __END__ >protein1 ASFGTHTRHTHRHTHTRHTRHTR >protein2 ERYRYTRYHTRHTGEFEWWFEEFFFFREFRGRE >protein3 AWEERERGRGRGREGRGREGRRRRRRRRTTHTHTRHRHTRHTR >protein2 AASEFEFEFE >protein4 REYTRHTRGRVEVCREVR