#substring(s) to search for: $substr = "AATGCTCCCCAATGAGGCA"; $substr2 = "TT##CACAAGGAACTTTTTT"; #Search for it in this string: $str = "CTTGAGTTATTAGCTGTTTGCCCCTGGGCAAGTTGTCTCACCTCTCCGTGTTTGTTTCCTCACCTATTGGACCAGGCCTATGGTTTTCAAACTGTGTTCCCTGGTGCTCTGAGACTTAGGGAAGGGGAACGCCCACACTGGCAAGGCTAACACAAGCCGCCATATACCCACCCCACTTCACCCACAGCACCCCCCTTTCTGTGTCTCATATACTGCGGGCCTATGCGA"; # the second substring is straightforward $pos = index( $string, $substr);