#!/usr/bin/perl
use strict;
use warnings;
my $qfn1 = "File1.txt";
my $qfn2 = "File2.txt";
my %positions;
{
open(my $fh, '<', $qfn1)
or die("Cannot open file \"$qfn1\": $!\n");
while (<$fh>) {
my ($key, $pos) = split /\s+/;
$positions{$key} = $pos;
}
}
my %sequences;
{
open(my $fh, '<', $qfn2)
or die("Cannot open file \"$qfn2\": $!\n");
my $key;
while (<$fh>) {
if ( s/^>// ) {
$key = ( split /\|/ )[1];
}
else {
chomp;
$sequences{$key} .= $_;
}
}
}
for my $key ( sort keys %positions ) {
if ( ! exists( $sequences{$key} )) {
warn "KEY $key in File1 not found in File2\n";
next;
}
if ( length( $sequences{$key} ) < $positions{$key} ) {
warn "KEY $key: File2 string length too short for File1 position value\n";
next;
}
my $index = rindex( $sequences{$key}, "ATG", $positions{$key} );
if ( $index < 0 ) {
warn sprintf( "KEY %s: No ATG in File2 string prior to position %d\n",
$key, $positions{$key} );
next;
}
$index += 3 while ( ($index + 3) < $positions{$key} );
print "$key $positions{$key} " . substr($sequences{$key}, $index, 3) . "\n";
}
####
File 1:-
155369268 17 A
155369268 70 C
269212605 90 G
File 2:-
>gi|155369268|ref|NM_001100917.1| some text
AAACAATGTCGATTCTATGATGCGAACGCAGCATTTCAGGGACTGGAATGGGAGCTTACGGTTTTTTACG
ACAGAATCATCAATATCTTGGAAGAAAAAGAATGTTAAGAAATAACAAAACAATAATTATTAAGTACTTT
>gi|255369205|ref|XM_001716884.1| some text
AGACAAGCTTGTCCTGATGTTCCTTGCCCTGGCAGATGTTCAGGACCATGGGAGCTTACGGTTTTTTACG
CTAAATGGCCCAAGCTTTCGGGGCTGTCATTGTCTGTTTGTCATTCAAGGGCCCAAGCTGAAGAGGGGGT
>gi|269212605|ref|XM_401716884.1| some text
AGACAAGCTTGTCCTGATGTTCCTTGCCCTGGCAGATGTTCAGGACCTTCCTTTGATTCAACCCTATGAC
CTAATTGGCCCAAGCTTTCGGGGCTGTCATTGTCTGTTTGTCATTCAAGGGCCCAAGCTGAAGAGGGGGT
####
155369268 17 CTA
155369268 70 CGC
269212605 120 GGG
but sadly my output is coming like this :(
155369268 70 CGC
269212605 120 GGG