#! usr/bin/perl
use strict;
use warnings;

# opening the input lexicons

open (GENE,"/home/stanley/Desktop/Gene.txt");
open (TARGET, "/home/stanley/Desktop/Target.txt");

my $target;
my $gene;

# opening fulltext

open (IF, "/home/stanley/Desktop/18048320.txt");
my $text = <IF>;
my @splittext = split (/[.] [A-Z]/, $text);
close (IF);

# opening output file

open (OF, ">/home/stanley/Desktop/Local.txt");

# Parsing Text

for $gene (<GENE>) {

chomp $gene;
	while ($target = <TARGET>) {
	chomp $target;

			foreach my $line (@splittext) {
				if ($line =~ /.+?($gene).*(localizes to|held|located in|localization|translocated to|targets|reaches|exported|export).*($target).+?/ig) {
				print OF $1."\t".$2."\t".$3."\n";
				}
			}
	
	}
} 
close (OF);
close (GENE);
close (TARGET);

##</code><code>##

____DATA___

gene.txt

pfrom1
pfama1
ama1
ha-pfrom1

target.txt

apicoplast
mitochondrion
rhoptry
rhoptries
golgi
dense granules
parasitophorous vacuole
micronemes
food vacuole
secretory vesicle
host cell

input txt
ompartmentalization of proteins into subcellular organelles in eukaryotic cells is a fundamental mechanism of regulating complex cellular functions. Many proteins of Plasmodium falciparum merozoites involved in invasion are compartmentalized into apical organelles. We have identified a new merozoite organelle that contains P. falciparum rhomboid-1 (PfROM1), a protease that cleaves the transmembrane regions of proteins involved in invasion. By immunoconfocal microscopy, PfROM1 was localized to a single, thread-like structure on one side of the merozoites that appears to be in close proximity to the subpellicular microtubules. Using antibodies to the merozoite surface protein-1 (MSP1), a protein that is located in the merozoite plasma membrane (Fig. 3A), we demonstrated that HA-PfROM1 staining is intracellular, not colocalizing with the plasma membrane. In merozoites, PfAMA1 is located in micronemes and thus separated from PfROM1. Toxoplasma gondii ROM1, the orthologue of PfROM1, is located in the Golgi, secretory vesicles, and in micronemes (10; L. D. Sibley, unpublished data). For example, Plasmodium falciparum apical membrane antigen 1 (AMA1) is held in the micronemes in merozoites inside of erythrocytes. For example, Plasmodium falciparum apical membrane antigen 1 (AMA1) is held in the micronemes in merozoites inside of erythrocytes.