____MY RESULTS____ PfROM1 localized subpellicular microtubules By immunoconfocal microscopy, PfROM1 was localized to a single, thread-like structure on one side of the merozoites that appears to be in close proximity to the subpellicular microtubules. PfROM1 localized subpellicular microtubules HA-PfROM1 was observed to be localized in close proximity to longitudinal subpellicular microtubules of the merozoite (Fig. PfROM1 localized rhoptries Thus, these results indicate that HA-PfROM1 is localized in a subcellular compartment distinct from the micronemes, rhoptries, and dense granules. PfROM1 localized rhoptries HA-PfROM1 is not localized to known apical secretory organelles: rhoptries, micronemes, and dense granules. PfROM1 located in Golgi Toxoplasma gondii ROM1, the orthologue of PfROM1, is located in the secretory vesicles, Golgi, and in micronemes (10; L. D. Sibley, unpublished data). PfROM1 localized dense granules Thus, these results indicate that HA-PfROM1 is localized in a subcellular compartment distinct from the micronemes, rhoptries, and dense granules. PfROM1 localized dense granules HA-PfROM1 is not localized to known apical secretory organelles: rhoptries, micronemes, and dense granules. PfROM1 localized micronemes Thus, these results indicate that HA-PfROM1 is localized in a subcellular compartment distinct from the micronemes, rhoptries, and dense granules. PfROM1 localized micronemes HA-PfROM1 is not localized to known apical secretory organelles: rhoptries, micronemes, and dense granules. PfROM1 localized micronemes HA-PfROM1 staining appeared to be colocalized, in part, with the PfAMA1 staining that translocates from micronemes to the parasite surface on release of micronemal contents during invasion (Fig. PfROM1 located in micronemes Toxoplasma gondii ROM1, the orthologue of PfROM1, is located in the secretory vesicles, Golgi, and in micronemes (10; L. D. Sibley, unpublished data). PfROM1 located in micronemes PfROM1 was also thought to be located in micronemes (13), based on data localizing a PfROM1 construct that was missing two 5′ exons which encode one of the transmembrane domains of PfROM1 (SI Figs. PfROM1 located in secretory vesicle Toxoplasma gondii ROM1, the orthologue of PfROM1, is located in the secretory vesicles, Golgi, and in micronemes (10; L. D. Sibley, unpublished data). HA-PfROM1 localized subpellicular microtubules HA-PfROM1 was observed to be localized in close proximity to longitudinal subpellicular microtubules of the merozoite (Fig. HA-PfROM1 localized rhoptries Thus, these results indicate that HA-PfROM1 is localized in a subcellular compartment distinct from the micronemes, rhoptries, and dense granules. HA-PfROM1 localized rhoptries HA-PfROM1 is not localized to known apical secretory organelles: rhoptries, micronemes, and dense granules. HA-PfROM1 localized dense granules Thus, these results indicate that HA-PfROM1 is localized in a subcellular compartment distinct from the micronemes, rhoptries, and dense granules. HA-PfROM1 localized dense granules HA-PfROM1 is not localized to known apical secretory organelles: rhoptries, micronemes, and dense granules. HA-PfROM1 localized micronemes Thus, these results indicate that HA-PfROM1 is localized in a subcellular compartment distinct from the micronemes, rhoptries, and dense granules. HA-PfROM1 localized micronemes HA-PfROM1 is not localized to known apical secretory organelles: rhoptries, micronemes, and dense granules. HA-PfROM1 localized micronemes HA-PfROM1 staining appeared to be colocalized, in part, with the PfAMA1 staining that translocates from micronemes to the parasite surface on release of micronemal contents during invasion (Fig. AMA1 translocated to apicoplast The protein AMA1 is then translocated to the food vacuole, apicoplast, subpellicular microtubules. AMA1 translocated to subpellicular microtubules The protein AMA1 is then translocated to the food vacuole, apicoplast, subpellicular microtubules. AMA1 held micronemes For example, Plasmodium falciparum apical membrane antigen 1 (AMA1) is held in the micronemes in merozoites inside of erythrocytes. AMA1 located in micronemes In merozoites, PfAMA1 is located in micronemes and thus separated from PfROM1. AMA1 translocated to food vacuole The protein AMA1 is then translocated to the food vacuole, apicoplast, subpellicular microtubules.