my $first_counter = 0; my $second_counter = 0; my $sequence = "GGGGGGGAGAAAAAAAAAAAAAAAGAAGGA"; # This is what I initially did, but did not get the right answer $first_counter++ while $sequence =~/AAAAA/g; #Here I get 3 # Because I want to count the overlapping AAAAA in the sequence I hunted around and found this solution $sequence =~ /AAAAA(?{$second_counter++})(?!)/; # This gives the right answer of 11 print "First counter: $first_counter\n"; print "Second counter: $second_counter\n";