#!/usr/bin/perl use warnings; use strict; # open file my $file = shift @ARGV; open FILE1, '<', $file or die "Can't open '$file': $!"; my %caseCount; # Use a while loop to read through the data while ( ) { my $caseNumber = ( split /\t/ )[ 1 ]; $caseCount{ $caseNumber }++; } # close the file you don't need it any more close FILE1; #do something here with values in %caseCount