use strict; use warnings; use Bio::SeqIO; my $file = "FastA.txt"; my $seq_obj = Bio::SeqIO->new(-file =>"<$file",-format=>'fasta',); my %seqPair; my %seq_NoPair; while(my $seq = $seq_obj->next_seq){ #iterate through records if($seq->desc =~ /.*?pair.*?/i){ $seqPair{$seq->id}=$seq->seq; }else{ $seq_NoPair{$seq->id}=$seq->seq; } } #use Data::Dump qw(pp); #print pp \%seqPair; #print pp \%seq_NoPair;