#!/usr/bin/perl -w use strict; while () { next if /^\s*$/; #skip blank lines my ($input, $CIGAR) = split; my $ref = $input; #working copy of $input my (@edit_cmd) = $CIGAR =~ m/\d+\w/g; my $curr_pos = 0; my $total_len =0; foreach my $cmd (@edit_cmd) { if (my ($M) = $cmd =~ m/(\d+)M/) { $curr_pos += $M; $total_len+= $M; } elsif (my ($I) = $CIGAR =~ m/(\d+)I/) { substr($ref,$curr_pos,$I,''); #delete $I characters $total_len -= $I } elsif (my ($D) = $CIGAR =~ m/(\d+)D/) { substr($ref,$curr_pos,0,"X" x $D); #insert $D X's $total_len += $D; $curr_pos += $D; } } $ref = substr($ref,0,$total_len); #truncate ????? print "INPUT = $input CIGAR = $CIGAR\n"; print "REF = $ref\n\n"; } =prints INPUT = CGAATTAATGGGAATTG CIGAR = 8M2I7M REF = CGAATTAAGGAAT INPUT = CGAATTAATGGGAATTG CIGAR = 2M2I2M3D10M REF = CGTTTGGGAA INPUT = CGAATTAATGGGA CIGAR = 8M2D7M REF = CGAATTAAXXTGGGA =cut __DATA__ CGAATTAATGGGAATTG 8M2I7M CGAATTAATGGGAATTG 2M2I2M3D10M CGAATTAATGGGA 8M2D7M