#!/usr/bin/perl
use strict;
use warnings;
use Text::CSV;

my @result;
my $csv = Text::CSV->new ( { sep_char => ' ' } )  # should set binary attribute.
                or die "Cannot use CSV: ".Text::CSV->error_diag ();
 
open my $fh, "<", "test.csv" or die "test.csv: $!";
while ( my $row = $csv->getline( $fh ) ) {
    if ($row->[0] =~ m{\QHWUSI-EAS95L_0025_FC:3:1:5232:1082#0/\E}) {
        if (@result) {
            $result[1] = $row->[3];
        } else {
            $result[0] = $row->[2];
        }
    }
}
printf "%s\t%s\n", @result;
$csv->eof or $csv->error_diag();
close $fh;