>3841	29	58.786702607127	GAGTAGTTCATAATAAAGAGGAGGCTGGT	
>3841	28	58.3143841442903	AGTAGTTCATAATAAAGAGGAGGCTGGT	
>486	26	59.8238809443041	CATTTTTCCTGAGCGTTTTCCTGAGC	
>486	25	59.1450485783588	ATTTTTCCTGAGCGTTTTCCTGAGC	
>486	24	58.9556227674582	TTTTTCCTGAGCGTTTTCCTGAGC	
>486	23	58.6081353592492	TTTTCCTGAGCGTTTTCCTGAGC	
>486	22      58.2296488444454	TTTCCTGAGCGTTTTCCTGAGC	
>136	25	Tm=59.8064145079347	CGAACAGAGGTCTCATGGAGAAACG