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perl -i -pe's/^(ISA\~.*?\~.*?\~.*?\~.*?\~.*?\~.*?\~.*?\~)(..........)(....).(\~.*)$/$1${2}0$3$4/' PB385930.P0
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ssh ... 'perl -i -pe'\''s/^(ISA\~.*?\~.*?\~.*?\~.*?\~.*?\~.*?\~.*?\~)(..........)(....).(\~.*)$/$1${2}0$3$4/'\'' PB385930.P0'