############### read in blast table and parse ####### my $in_blast_tab=$ARGV[0]; open(IN,$in_blast_tab) or die "cannot open $in_blast_tab\n"; my $HoFlg1={}; my $HoFlg2={}; #my $Flag1; my $Flag2; my %maxBits; my $max_bits=60; while (my $line=) { chomp $line; my ($Query_id,$strand,$Subj_id,$Perc_iden,$align_len,$num_mm,$gap,$q_start,$q_end,$s_start,$s_end,$e_value,$bit_score)=split("\t",$line); # extra f ield of strand next if ($bit_score <60); if ($bit_score > $max_bits){ $max_bits=$bit_score; } $maxBits{$Query_id}=$max_bits; my ($Flag1, $Flag2 ) = &Flag( $Subj_id, \%proph_prots, \%euk_prots, \%vir_prots ); $HoFlg1->{$Query_id}->{$Flag1}->{$bit_score}++; $HoFlg2->{$Query_id}->{$Flag2}->{$bit_score}++; print join("\t",$Query_id,$Subj_id,$bit_score,$Flag1,$Flag2)."\n"; } ## Get a Flag for each query/subject sub Flag { my ( $Subj_id, $proph_prots, $euk_prots, $vir_prots ) = @_; return "Proph", "Phage" if exists $$proph_prots{$Subj_id}; return "Euk", "Euk" if exists $$euk_prots{$Subj_id}; return "Vir", "Phage" if exists $$vir_prots{$Subj_id}; return "Bact", "Bact"; } ## end sub Flag ## now for all query/flag pairs with bit scores that are within 90% of the top_bit for that query ## my @flag_list2=("Phage","Euk","Bact"); my $count={}; foreach my $q (keys %{$HoFlg2}){ print "$q\t"; for my $flag (@flag_list2){ for my $b (keys %{$HoFlg2->{$flag}}){ if ($b > 0.9*$maxBits{$q}){ $count->{$flag} += $HoFlg2->{$q}->{$flag}->{$b}; } } print "$count\t"; } print "\n"; } #### 158256496-stool1_revised_C972998_1_gene3 strand:- 581103.GY4MC1_2020 37.93 116 61 2 1 110 1 111 1e- 158256496-stool1_revised_C972998_1_gene3 strand:- 539329.FTPG_01302 36.28 113 71 1 1 113 1 112 1e- 158256496-stool1_revised_C972998_1_gene3 strand:- 634956.Geoth_2108 37.93 116 61 2 1 110 1 111 1e- V1.UC9-0_revised_scaffold1508_3_gene71913 strand:+ 565641.EFOG_02198 57.55 212 55 3 1 212 967 1143 9e- V1.UC9-0_revised_scaffold1508_3_gene71913 strand:+ 565640.EFMG_02145 57.55 212 55 3 1 212 967 1143 9e- V1.UC9-0_revised_scaffold1508_3_gene71913 strand:+ 565639.EFYG_01514 57.55 212 55 3 1 212 967 1143 9e- V1.UC9-0_revised_scaffold1508_3_gene71913 strand:+ 565638.EFCG_01989 57.55 212 55 3 1 212 967 1143 9e- #### 158256496-stool1_revised_C972998_1_gene3 HASH(0x2b3c2bd3b000) HASH(0x2b3c2bd3b000) HASH(0x2b3c2bd3b000) V1.UC9-0_revised_scaffold1508_3_gene71913 HASH(0x2b3c2bd3b000) HASH(0x2b3c2bd3b000) HASH(0x2b3c2bd3b000) #### #query num_Phage num_Euk num_Bact query_1 5 1 0 query_2 0 6 1