$genes = $ARGV[3]; $isoforms = $ARGV[4]; $trans = $ARGV[0]; $left = $ARGV[1]; $right = $ARGV[2]; $directory = "/home/applications/galaxy-dist/tools/VELVET-OASES"; $transcript = "$directory/trans.fa"; $left_read = "$directory/left_read.fastq"; $right_read = "$directory/right_read.fastq"; symlink $trans, $transcript; symlink $left, $left_read; symlink $right, $right_read; @out = `perl /home/applications/galaxy-dist/tools/VELVET-OASES/run_rsem.pl 2>&1`;