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  1. or download this
    LOC_Os01g01010.1 : PS00022 EGF_1 EGF-like domain signature 1.
          20 - 31  CtCtaAgaGAaC                                           
    +      L=(-1)
    ...
        463 - 520  CaacCTCcagcgcggcgttggCtcc.CtcgtccgtgaCattggcgacccctg..C
    +CtcaaC L=(-1)
        484 - 529  CtccCTCgtccgtgacattggCgaccCctgc......Ctcaacccat.......C
    +Ccc..C L=(-1)
    
  2. or download this
    #!/usr/local/bin/perl
    use strict;
    ...
    close (MYFILE);
    close (WASTE);
    close (FILE);