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Use of uninitialized value in concatenation (.) or string in line 1245

for (my $s=$start_of_barrel_region; $s<=(($start_of_barrel_region+$len
+gth_of_barrel_region)); $s++)
{
    my $aa_pos=$s+1;
    print PLP_FILE   "$aa_pos\t".$split_query_for_PLP[$s]."\t".$hash_p
+lp_lbl_barrel_region_only{$s}."\n";    
}

my $aa_pos=$s+1;