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use warnings;
use strict;
...
print "SECOND Connection with dbfile read from file $dbfile2 ... ";
$dbh = DBI->connect("dbi:SQLite:dbname=$dbfile2","","")|| die $DBI::er
+rstr;
print "done!\n";

FIRST Connection to dbfile path read from script (C:/users/DON/Desktop
+/data.db) ... done!

Reading dbfile path from configuration file... C:/users/DON/Desktop/da
+ta.db
Can't connect to data source 'dbi:SQLite:dbname=C:/users/DON/Desktop/d
+ata.db' because I can't work out what driver to use (it doesn't seem 
+to contain a 'dbi:driver:' prefix and the DBI_DRIVER env var is not s
+et) at script/myscript.pl line 22.
SECOND Connection with dbfile read from file C:/users/DON/Desktop/data
+.db ...