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DBD::mysql:st execute failed: Column count doesn't match value count a
+t row 1 at dbtest.pl line 22

#!perl -w
use strict;
...
}

$DBH->disconnect;

create table Questions(Test_Number integer(2) NOT NULL,
                       Question varchar(255) NOT NULL,
...
                       Answer_D varchar(255),
                       Answer_E varchar(255),
                       Correct_Answer varchar(10));