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# load data into a list of hash-refs
my @data = (
  { Subject => 'ID12', Query => 'KBrH', start => 22400316, ... },
...
my @sorted = sort {
  $a->{Subject} cmp $b->{Subject} or $a->{start} <=> $b->{start}
} @data;

use Data::Sorting 'sort_array';
sort_array( @data, 'Subject', 'start' );

my $data = [
  [ 'ID12', 'KBrH', '2e-26', 22400316, ... ],
  ...
...

use Data::Sorting 'sort_arrayref';
sort_arrayref( $data, 0, 3 );

use Data::Sorting 'sorted_array';
my @sorted = sorted_array( @data, 'Subject', 'start' );