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<prefix:monastery xmlns:prefix="http://perlmonks.org">
  <prefix:foo>
...
    </prefix2:bar>
  </prefix:foo>
</prefix>

my $trigger = "prefix:monastery/prefix:foo/prefix2:bar/prefix2:monk";
my $twig = XML::Twig->new(TwigHandlers => { $trigger => \&function });

my $trigger = "*:monastery/*:foo/*:bar/*:monk";
my $twig = XML::Twig->new(TwigHandlers => { $trigger => \&function });

my $xml_ns = {
"http://perlmonks.org" => prefix,
...
  map_xmlns => $xml_ns,
  TwigHandlers => { $trigger => \&function }
);

use XML::Twig;
use strict;
...
XML

$twig->parse($xml);

use XML::Twig;
...
XML

$twig->parse($xml);