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#!/usr/bin/perl -w
use strict;
...
   }

}

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Trial 1
1 : TGCAATCACTAGCAAGCTCTCGCTGCCGTCACTAGCCTGTGG
...
seek() on closed filehandle INFILE at test.pl line 10.
Trial 2
readline() on closed filehandle INFILE at /usr/lib/perl5/site_perl/5.8
+.0/Bio/Root/IO.pm line 440.