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N01A0000.f BG1_c22
N01A000X.f BG1_c5
...
N01A00F3.f BG1_c14
N01A00FX.f BG1_c7
N01A00FX.r BG1_c7

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open(FILE,"/Downloads/samplefile.txt") or die $!;
while(<FILE>){
...
        }

}