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 #! usr/bin/perl
use strict;
use warnings;
...
close (OF);
close (GENE);
close (TARGET);

____DATA___

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input txt
ompartmentalization of proteins into subcellular organelles in eukaryo
+tic cells is a fundamental mechanism of regulating complex cellular f
+unctions. Many proteins of Plasmodium falciparum merozoites involved 
+in invasion are compartmentalized into apical organelles. We have ide
+ntified a new merozoite organelle that contains P. falciparum rhomboi
+d-1 (PfROM1), a protease that cleaves the transmembrane regions of pr
+oteins involved in invasion. By immunoconfocal microscopy, PfROM1 was
+ localized to a single, thread-like structure on one side of the mero
+zoites that appears to be in close proximity to the subpellicular mic
+rotubules. Using antibodies to the merozoite surface protein-1 (MSP1)
+, a protein that is located in the merozoite plasma membrane (Fig. 3A
+), we demonstrated that HA-PfROM1 staining is intracellular, not colo
+calizing with the plasma membrane. In merozoites, PfAMA1 is located i
+n micronemes and thus separated from PfROM1. Toxoplasma gondii ROM1, 
+the orthologue of PfROM1, is located in the Golgi, secretory vesicles
+, and in micronemes (10; L. D. Sibley, unpublished data). For example
+, Plasmodium falciparum apical membrane antigen 1 (AMA1) is held in t
+he micronemes in merozoites inside of erythrocytes. For example, Plas
+modium falciparum apical membrane antigen 1 (AMA1) is held in the mic
+ronemes in merozoites inside of erythrocytes.