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 if(/;;HPLCColumn;;([0-9]+);/){
                            if ($number == 2){
                             $HPLC_Collum= $1;
...
                        }else {
                           die ; 
                        }

sub capconverter {
    my($excelfile ,$HPLC_to_UV_Factor ,$HPLC_Collum ,$UV_Collum ,$Data
+_row_start)=@_;
...
$Book->Close;
 }

Win32::OLE(0.1709) error 0x800a03ec
    in METHOD/PROPERTYGET "Cells" at C:\Documents and Settings\KHusban
+d\Desktop\
New Folder\exceel.pl line 272