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open (FILE,"abc345.txt") or die $!;
       while (<FILE>){
       $_ = /((^Query = .*)|(^\>.*)|(Length = .*))/i;
...
      
   }
  close (FILE) ;

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open (FILE,"abc345.txt") or die $!;
       while (<FILE>){
       $_ = /((^Query = .*)|(^\>.*)|(Length = .*))/i;
...
      
   }
  close (FILE) ;