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    # V5: Inline::C
    #!/opt/local/bin/perl
    ...
        ( $ref, $matches, $query ) = $read->padded_alignment;
        print $read->qname, "\n", dnacmp2('$ref', '$query');
    }
    
  2. or download this
    
    Global symbol "$query" requires explicit package name at mutations.pl 
    +line 133.
    ...
    Global symbol "$matches" requires explicit package name at mutations.p
    +l line 133.
    Global symbol "$query" requires explicit package name at mutations.pl 
    +line 133.
    Execution of mutations.pl aborted due to compilation errors.