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  1. or download this
    my $open;         # variable with file scope
# The sub open :
sub open_file {$open = $mw->getOpenFile(    # no my here!
   ...
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    sub get_statistics {
    ...
    my $fastaFile = $open;
    ...
  3. or download this
    sub get_statistics {
    ...
    my $fastaFile = $current_file;
    ...