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  1. or download this
    #!/usr/local/bin/perl
    use warnings;
    use strict;
    ...
    use Bio::Seq;
    use Bio::Tools::Primer3;
    use Data::Dumper;
    
  2. or download this
    my $seqio = new Bio::SeqIO( 
       -file => "<(PRIVATE)/fastaUplist.fa",                              
    +  
       -format =>'fasta');
    ...
    
    my $argref = $primer3->arguments();
    print Dumper($argref);