You've got 2 problems - which one do you want help with? the DBD::Sybase problem or the DBD::ODBC one? For the DBD::Sybase one you should check your Sybase interfaces file I think (but I'm not a Sybase guy so I could be wrong). For the ODBC issue I can help but I'll need to know what ODBC driver you are using. The odbc.ini file is irrelevant if you are using a DSN-less connection which you are if your ODBC connection string starts with DRIVER={something} instead of DSN=somethingelse. I've already shown you a valid odbcinst.ini file.