in reply to Re^2: find reverse complement of every 2nd line
in thread find reverse complement of every 2nd line

my problem actually is doing this only to every other line and not the entire file

The special variable $. (documented in perldoc perlvar) keeps count of the line numbers, so you can do something like:
while(<IN>) { next if $. % 2; # skip odd lines # do the processing }
Cheers,
Rob