Re: genetic algorithm for motif finding
by marto (Cardinal) on Aug 13, 2013 at 08:42 UTC
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What part do you need help with? Most people are happy to lend a hand and answer questions you have, few are willing to do your job and write the whole program for you. Some links which are worth reading and understanding:
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Re: genetic algorithm for motif finding
by bioinformatics (Friar) on Aug 13, 2013 at 21:31 UTC
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The original paper for FMGA (finding motifs by genetic algorithm) is here:
link. It gives a decent breakdown of the algorithm and provides pseudocode. What part(s) are you having difficulty coding? I'm not sure that someone is going to have the exact code you want lying around, but they can help you implement things.
EDIT: fixed the link
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A motif, also termed a consensus sequence, is a stretch of DNA that has either the exact (rare) or a similar sequence in many places across the genome; you can think of it like input to a fuzzy search, close but not exact, maybe a misspelling or two. These sites often serve as places where proteins physically interact and bind along the DNA. There are data collections, based on sequencing data, that allow one to know where along the DNA that a given protein binds, and from which one may look for enriched or common motifs by looking at the sequence from the binding coordinates. It may be that these motifs are associated with the protein in question, or they may be motifs for other proteins which interact with the protein you have data for. Collections of motifs in a region (such as the promoter region, where many proteins bind to turn a gene on, off, increased, or decreased--think dimmer switch) can be refered to as cis regulatory regions. You know you found one when you can see an enrichment or increased frequency over some background (control) sequence. Common programs used in this analysis are MEME and nestedMICA. Does this help a bit?
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You can learn a bit of bioinformatics at Rosalind. By solving problems, you not only gain XP and contest, but also learn about the underlying science.
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Although the OP is interested in DNA, the term is also used for amino acids/proteins.
The Wikipedia entry for Sequence motif is pretty clear, I think.
Also enlightening may be: Sequence logo.
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Re: genetic algorithm for motif finding
by Anonymous Monk on Aug 13, 2013 at 06:57 UTC
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